一种制备鱼鳞胶原蛋白活性肽的方法

Preparation method for fish scale collagen protein active peptide

Abstract

本发明公开一种制备鱼鳞胶原蛋白活性肽的方法,涉及渔业加工副产品的开发利用领域。按照下述步骤进行:配制底物浓度为20 g/L的鱼鳞胶原蛋白溶液1000 mL,经聚能式逆流脉冲超声波预处理。取超声预处理后的鱼鳞胶原蛋白溶液置入酶膜反应器内,然后加入碱性蛋白酶酶解,酶解反应25 min后启动超滤循环装置,进行梯度稀释补料式酶膜耦合反应。反应结束后将透过液采用常规喷雾干燥或冷冻干燥即可得鱼鳞胶原蛋白活性肽。本发明采用超声辅助酶解-稀释梯度补料酶膜耦合反应技术制备鱼鳞胶原蛋白ACE抑制肽,有效的减少了产物抑制效应,进一步改善了酶解反应效果,提高了酶的利用度,降低了生产成本。
Relating to the field of development and utilization of fishery processing by-products, the invention discloses a preparation method for fish scale collagen protein active peptide. The method is carried out according to the steps of: preparing 1000mL of a fish scale collagen protein solution with a substrate concentration of 20g/L, conducting energy-gathered counterflow pulse ultrasonic pretreatment, putting the fish scale collagen protein solution subjected to ultrasonic pretreatment in an enzyme membrane reactor, then adding alkaline protease to perform enzymolysis, after 25min of enzymolysis reaction, starting an ultrafiltration circulating device to carry out gradient dilution feeding type enzyme membrane coupling reaction, and at the end of the reaction, subjecting the permeate liquid to conventional spray drying or freeze drying, thus obtaining the fish scale collagen protein active peptide. The method provided by the invention adopts the ultrasound-assisted enzymolysis-dilution gradient feeding enzyme membrane coupling reaction technology to prepare fish scale collagen protein ACE inhibitory peptide, effectively reduces the product inhibition effect, further improves the enzymolysis reaction effect, enhances the enzyme utilization degree, and reduces the production cost.

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    Title
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